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Contact Name
Sutiman Bambang Sumitro
Contact Email
berkalahayati@yahoo.com
Phone
+62341570631
Journal Mail Official
wulidanisa@berkalahayati.org
Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia
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Unknown,
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INDONESIA
Berkala Penelitian Hayati
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Articles 8 Documents
Search results for , issue "Vol 22 No 2 (2017): June 2017" : 8 Documents clear
The potency of Micrococcus sp. L II 61 bacteria as oil sludge cleaning agent Ni’matuzahroh Ni’matuzahroh; Intan Ayu Pratiwi; Tini Surtiningsih; Fatimah Fatimah; Sri Sumarsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.394 KB) | DOI: 10.23869/56

Abstract

This research aimed to reveal the ability of Micrococcus sp. L II 61 bacteria that was isolated from Pegirian Surabaya as oil cleaning agent. This is an experimental research to detect the presence of biosurfactant and lipase enzyme in culture supernatant of Micrococcus sp. L II 61 with aliphatic hy-drocarbon (cooking oil) as a substrate growth. Biosurfactant production was evaluated by measuring the surface tension of supernatant using tensiom-eter du Nouy and measuring the emulsification activity value using diesel oil as hydrocarbon test. Lipase enzyme was detected by measuring lipolitic activity value of crude enzyme (culture supernatant) by using p-nitrofenil palmitic (p-npp) as a substrate test. Data were analyzed descriptively. The results showed that Micrococcus sp. L II 61 produced biosurfactant with surface tension decreasing of culture supernatant up to 30.27 ± 1.17 mN/m compared than aquadest and value of hydrocarbon emulsification activity (AE 1 hour) up to 20.24 ± 0.68 %. Culture of Micrococcus sp. L II 61 after 16 hours incubation have a lipolytic activity 33.53 ± 0.14 U/mL at pH 7 and 37 oC. Supernatant of Micrococcus sp. L II 61 100% (v/v) give the high-est percentage of oil sludge solubility, i.e. 86.38 ± 2.39%. Micrococcus sp. L II 61 is a highly potential to be developed as oil sludge cleaning agent.
Molecular biology education in Indonesia - suggestions for improvement Wolfgang Nellen; Atika Hanoum Rahasta; Feri Eko Hermanto; Margareth Rika Wahyusari Gabriella Lorenza
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (825.118 KB) | DOI: 10.23869/59

Abstract

Education in molecular biology is considered difficult because it is complex and requires a lot of basic knowledge. Focused on basic science facts, it is usually taught with little connection to everyday life. A second problem is that the topic is rather abstract and results are only indirectly observed: we know that DNA is a double helix - but nobody has seen it directly. In contrast to subjects in the humanities and social sciences, molecular biology is a handy-craft where sophisticated theory and practical lab-work go hand in hand. Molecular biology is not so much a specific sub-discipline of biosciences but rather a huge tool box which is indispensable for all other fields of biology. It is therefore absolutely required for all biologists to know the basics and the potential of molecular biology in their specific field of interest. We present some thoughts how to make the theory more attractive, how to combine learning, training and teaching of theory and practice, how to stimulate independent, critical thinking in students and final-ly, how to identify and support excellent students who should advance science in the next generation. Last not least, we emphasize the necessity to expand information and teaching of molecular biology to the public.
Potential probiotic evaluation of two Lactobacillus plantarum strains isolated from Indonesian fermented food and fruit A’liyatur Rosyidah; Heddy Julistiono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (707.508 KB) | DOI: 10.23869/60

Abstract

Two Lactobacillus plantarum strains, InaCC B122 and InaCC B128, isolated from Indonesian fermented food, “dadih” and fruit were evaluated for probiotic potential. These two lactobacilli strains were subjected to a series of in vitro test to assess their surviving capability in gastrointestinal track and antimicrobial activity. The test included acid and bile salt tolerance, adhesion to the yeast Saccharomyces cereviseae cells, heat resistance and antimicrobial activity against Mycobacterium smegmatis, Escherichia coli, and Staphylococcus aureus. Both strains were able to grow under acid and bile salt condition for 24 h. Co-aggregation percentage of strain InaCC B122 and InaCC B128 against the yeast cells was 52.3 % and 53.8 % respec-tively. Heat treatments (45 ℃ for 30 min. and 55 ℃ for 15 min.) did not affect co-aggregation percentages. Their supernatants had antimicrobial activity against M. smegmatis, E. coli and S. aureus with MIC of 5 %, 10 % and 10 % respectively. In conclusion, based on this assessment, these strains are good candidate for probiotic.
The utilization of ink cap mushroom (Coprinus cinereus) on palm oil mill effluent degradation Yohanes Bernard Subowo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.433 KB) | DOI: 10.23869/64

Abstract

A research on the utilization of ink cap mushroom (Coprinus cinereus) on Palm Oil Mill Effluent (POME) degradation was conducted. Palm oil in-dustry is an agricultural industry which keeps on developing in Indonesia. Aside from producing palm oil, this industry also generates POME. POME has high level of BOD and COD value thus it is dangerous for the environment. Coprinus cinereus was examined to degrade POME because of its ability to produce ligninases enzymes. The aim of this research was to evaluate ligninases enzymes produced by C. cinereus after the addition of inducers and its ability in degrading POME. Coprinus cinereus was cultivated on Defined Medium (DM) then the mycelia was harvested Inducers used in this study were CuSO4, KNO3 and sucrose. The suspension of Coprinus cinereus mycelia was used to degrade POME. The result showed that Coprinus cinereus could produce Laccase, Mangan Peroxidase and Lignin Peroxidase on DM. The addition of 200 μM CuSO4 and 10 mM KNO3 on DM could increase laccase activity by 62.92 %. Coprinus cinereus has ability to degrade Poly R-478 and POME. The treatment of 200 μM CuSO4 on media could increase the degradation of Poly R-478 by 19.32% after 9 days of incubation. The addition of 15 g/L sucrose on POME could increase decolorization by 75.26 % and COD reduction by 91.26 % after 27 days of incubation. Ink Cap Mushroom (Coprinus cinereus) can be used for POME degradation. The addition of sucrose and heating treatment would accelerate COD reduction.
Characterization of biocontrol agents isolated from the leaves of mangroves grown in Sampiran Beach, North Sulawesi, Indonesia againts damping-off of cucumber Yuliar Yuliar; Suciatmih Suciatmih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.544 KB) | DOI: 10.23869/65

Abstract

Bacterial biocontrol isolates MND61, MND63, and MND65 were originally isolated from mangrove leaves of Derris trifoliata Lour, Sonneratia alba J. Smith, and Avicennia marina (Forsk) respectively grown in Sampiran beach, North Manado, North Sulawesi island, Indonesia. They were evaluated on suppression of cucumber damping-off. Identification results showed that based on 16S rRNA sequence, MND61 isolate had 99% similarity to Kleibsiella pneumoniae ATTCC13884T, MND63 isolate had 98% similarity to Bacillus cereus NENA11, and MND65 isolate had 98% similarity to Bacillus subtilis jllsy. Results of greenhouse test showed that MND61 and MND63 suppressed Pythium aphanidermatum damping-off of cucumber significantly (P < 0.005) by 73-80%. Cocultures of these two biocontrol agent in potato dextrose broth also suppressed the pathogens population about 5-46 %. MND61 isolate produced indole acetic acid, in contrary to MND63 and MND65 isolates did not. The inhibitory assay effect of broth culture of MND61, MND63, and MND65 isolates to phytopatogenic fungi Fusarium oxysporum, P. aphanidermatum, P. ultimum, Rhizoctonia solani, Pyricularia oryzae and Xanthomonas oryzae in PDA plate indicated that two strains (MND63 and MND65) inhibited the growth of F. oxysporum, and R. solani growth, otherwise MND61 isolate did not inhibit the growth of any phytopatogenic assay.
Carbon storage of medium-sized tree: a case study on Dillenia collection in Purwodadi Botanic Garden Ridesti Rindyastuti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (752.026 KB) | DOI: 10.23869/66

Abstract

Dillenia is a medium-sized tree which has high species diversity in tropical regions especially in Southeast Asia. Dillenia in Purwodadi Botanic Garden are collected from native habitats in Java, Kalimantan, Sulawesi and Papua which planted on the area of 17 x 55 m2. The purpose of this re-search is to study the above ground carbon storage in Dillenia collection in Purwodadi Botanic Garden. Carbon storage estimation was established by measuring stem carbon stocks from plant collections with plant age ranging between 12-30 years. Twelve years old collection contributed carbon storage of 7.35 tonnes/ha for D. sumatrana. Twenty years old species had the lowest carbon storage of 2.17 kg/plant for D. serrata and the highest of 51.9 kg/plant for D. auriculata with a range of carbon storage of 3.47 to 41.072 tonnes/Ha. Thirty years old plant contributed 39.465 kg/plant and carbon storages of 63.14 tonnes/ha for D. serrata and 135.59 kg/plant and 216.94 tonnes/ha for D. philipinensis. Overall, Dillenia collections in Pur-wodadi Botanic Garden contributed 793.94 kg carbon storages, store carbon on average of 30.54 kg/plant and 46.46 tonnes/ha. The increase of carbon storage in the second 10 years was higher than in the first 10 years. It indicated that Dillenia had growth strategy in the early growth then alocated more mass after 10 years. Carbon storage of Dillenia was high and different in age. D. serrata, D. papuana and D. auriculata are recommended species as a priority in planting trees based on carbon sink.
Crab communities (Decapoda: Brachyura) in mangrove and estuaries in the Eastern Part of Lombok Island Dewi Citra Murniati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1477.027 KB) | DOI: 10.23869/67

Abstract

Mangroves and estuary ecosystem in East Lombok which are appointed as Local Marine Conservation Areas (Kawasan Konservasi Laut Daerah/KKLD) are Gili Lawang, Gili Sulat, Sambelia and Jerowaru districts. Inventory of aquatic fauna in mangroves and estuaries is one of the important components of the marine conservation program. The objective of this study is to determine crab species composition in mangroves and estuaries of eastern Lombok during the East Lombok KKLD program. Targeted area for this study includes Gili Lawang, Gili Sulat in southern Lombok Island, Sugian in Sambelia, Ekas Beach, Tanjung Luar and Seriwe in Jerowaru, and Kidang in western Jerowaru. The crab was collected at 0 m to 100 m of seashore by digging the burrow. The crab community recorded was analyzed for resulting the Diversity, Evenness and Similarity Index. Ten families with 35 species of crab were found from two ecosystem types, mangrove and estuary. Each location has different diversity of ecosystem and habitat. The highest diversity is recorded in Kidang and Seriwe, while the lowest diversity in Gili Sulat. Species diversity is associated with ecosystem and habitat type diversity. Kidang and Seriwe has the highest ecosystem and habitat diversity, while Gili Sulat has the lowest diversity. In addition, the population of Ocypodidae is accounted as the dominant crab family for which Austruca perplexa is the dominant species.
Water quality and fish diversity in the Brantas River, East Java, Indonesia Alfiah Hayati; Nureka Tiantono; Muhamad Fadhil Mirza; Iman Dary Supriyadi Putra; Muhamad Maulana Abdizen; Antien Rekyan Seta; Binti Mar`atus Solikha; Muhamad Hilman Fu'adil; Trisnadi Widyaleksono Catur Putranto; Mochamad Affandi; Rosmanida Rosmanida
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (701.153 KB) | DOI: 10.23869/100

Abstract

This research aimed to determine the water quality and fish diversity in Brantas river. Three station for sampling locations were in the upstream (one station on Karangkates reservoir) and downstream (two stations on the Surabaya and Jagir river). Water quality parameters were BOD, COD, DO, pH, temperature, and the levels of heavy metals (Pb, Cr, Cu, and Cd). Fish specimens was identified using fish identification book. The results of this research indicated that there was a difference in the water quality of upstream and downstream of the Brantas river. On the upstream, the source of pollution was mostly from fish catching, agriculture, and industrial activities. While on the downstream of the river it mostly dominated by industrial waste and domestic waste. The water quality in both stations had far exceeded the quality standards that was included in the polluted category. The concluded that Brantas river has been contaminated by waste (heavy metals), exceeded the water quality standard. The diversity of fish in the downstream (Surabaya river) has the highest diversity values, followed Jagir river and in the upstream (Karangkates reservoir) has the lowest value of diversity. Brantas river water was included in the category of moderate diversity.

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